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横浜市立大学大学院生命医科学研究科生命医科学専攻分子細胞医科学研究室ホームページ:メンバー紹介 横浜市立大学大学院生命医科学研究科生命医科学専攻分子細胞医科学研究室ホームページ:メンバー紹介

横浜市立大学大学院生命医科学研究科生命医科学専攻分子細胞医科学研究室ホームページ:メンバー紹介

 MCBL:メンバーリスト


事務補佐員メンバー紹介

●大山 良文  (Yoshifumi Ohyama) 
杉原 文徳  (Fuminori Sugihara) 
●大槻 和重  (Kazushige Ohtsuki) 
●高橋 宏之  (Hiroyuki Takahashi) 
●黒川 裕美子 (Yumiko Kurokawa)
●村山 泰斗  (Yasuto Murayama)
●森 沙織   (Saori Mori )

●赤松 由布子 (Yufuko Akamatsu )
●加藤 由起  (Yuki Kato )
●高畑 信也  (Shinya Takahata )
●小林 晶子  (Akiko Kobayashi )
●月橋 美博  (Yoshihiro Tsukihashi )
●小谷 公泰  (Tomohiro Kotani )



  氏名:大山 良文 (2010.12.博士号取得@横浜市立大学)
  論文タイトル:出芽酵母のSsd1pはCLN2 mRNAの5'-非翻訳領域に結合しCLN2遺伝子の発現を促進する
  発表論文:
 

Kato Y, Kawasaki H, Ohyama Y, Morishita T, Iwasaki H, Kokubo T, Hirano H.
Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC/KEOPS Complex.
Genetics. 2011 Aug;188(4):871-82. Epub 2011 May 30.

Kasahara K, Ohyama Y, Kokubo T.
Hmo1 directs pre-initiation complex assembly to an appropriate site on its target gene promoters by masking a nucleosome-free region.
Nucleic Acids Res. 2011 May 1;39(10):4136-50. Epub 2011 Feb 2.

Ohyama Y, Kasahara K, Kokubo T.
Saccharomyces cerevisiae Ssd1p promotes CLN2 expression by binding to the 5'-untranslated region of CLN2 mRNA.
Genes Cells. 2010 Dec;15(12):1169-88. doi: 10.1111/j.1365-2443.2010.01452.x. Epub 2010 Oct 26.

Kobayashi A, Miyake T, Ohyama Y, Kawaichi M, Kokubo T.
Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae.
J Biol Chem. 2001 Jan 5;276(1):395-405.


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  氏名:杉原 文徳 (2010.12.博士号取得@横浜市立大学)
  論文タイトル:出芽酵母のRPS5遺伝子に由来するTATAレスプロモーターのシス配列解析
  発表論文:
 

Sugihara F, Kasahara K, Kokubo T.
Highly redundant function of multiple AT-rich sequences as core promoter elements in the TATA-less RPS5 promoter of Saccharomyces cerevisiae.
Nucleic Acids Res. 2011 Jan 1;39(1):59-75. Epub 2010 Aug 30.

Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI.
Angew Chem Int Ed Engl. 2009;48(20):3641-3.

Mizukami S, Takikawa R, Sugihara F, Hori Y, Tochio H, Wa¨lchli M, Shirakawa M, Kikuchi K.
Paramagnetic relaxation-based 19f MRI probe to detect protease activity.
J Am Chem Soc. 2008 Jan 23;130(3):794-5. Epub 2007 Dec 23.

Ki S, Sugihara F, Kasahara K, Tochio H, Shirakawa M, Kokubo T.
Magnetic resonance-based visualization of gene expression in mammalian cells using a bacterial polyphosphate kinase reporter gene.
Biotechniques. 2007 Feb;42(2):209-15.

Ki S, Sugihara F, Kasahara K, Tochio H, Okada-Marubayashi A, Tomita S, Morita M, Ikeguchi M, Shirakawa M, Kokubo T.
A novel magnetic resonance-based method to measure gene expression in living cells.
Nucleic Acids Res. 2006 Apr 5;34(6):e51.


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  氏名:大槻 和重 (2010.6.博士号取得@横浜市立大学)
  論文タイトル:出芽酵母における全TFIIDサブユニットのゲノムワイドなDNA結合解析
  発表論文:
 

Ohtsuki K, Kasahara K, Shirahige K, Kokubo T.
Genome-wide localization analysis of a complete set of Tafs reveals a specific effect of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different TFIID conformations at different promoters.
Nucleic Acids Res. 2010 Apr;38(6):1805-20. Epub 2009 Dec 21.

Kasahara K, Ohtsuki K, Ki S, Aoyama K, Takahashi H, Kobayashi T, Shirahige K, Kokubo T.
Assembly of regulatory factors on rRNA and ribosomal protein genes in Saccharomyces cerevisiae.
Mol Cell Biol. 2007 Oct;27(19):6686-705. Epub 2007 Jul 23.

Takahata S, Ryu H, Ohtsuki K, Kasahara K, Kawaichi M, Kokubo T.
Identification of a novel TATA element-binding protein binding region at the N terminus of the Saccharomyces cerevisiae TAF1 protein.
J Biol Chem. 2003 Nov 14;278(46):45888-902. Epub 2003 Aug 25.


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  氏名:高橋 宏之 (2008.12.博士号取得@横浜市立大学)
  論文タイトル:出芽酵母におけるメディエーターサブユニットMed9の機能解析
  発表論文:
 

Takahashi H, Kasahara K, Kokubo T.
Saccharomyces cerevisiae Med9 comprises two functionally distinct domains that play different roles in transcriptional regulation.
Genes Cells. 2009 Jan;14(1):53-67. Epub 2008 Dec 11.

Kasahara K, Ki S, Aoyama K, Takahashi H, Kokubo T.
Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase II.
Nucleic Acids Res. 2008 Mar;36(4):1343-57. Epub 2008 Jan 10.

Kasahara K, Ohtsuki K, Ki S, Aoyama K, Takahashi H, Kobayashi T, Shirahige K, Kokubo T.
Assembly of regulatory factors on rRNA and ribosomal protein genes in Saccharomyces cerevisiae.
Mol Cell Biol. 2007 Oct;27(19):6686-705. Epub 2007 Jul 23.


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  氏名:黒川 裕美子 (2008.3.博士号取得@大阪大学)
  論文タイトル:分裂酵母におけるDNA相同組換え反応の分子機構
  発表論文:
 

Kurokawa Y, Murayama Y, Haruta-Takahashi N, Urabe I, Iwasaki H.
Reconstitution of DNA Strand Exchange Mediated by Rhp51 Recombinase and Two Mediators.
PLoS Biol. 2008 Apr 15;6(4):e88

Murayama Y, Kurokawa Y, Mayanagi K, Iwasaki H.
Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases.
Nature. 2008 Feb 21;451(7181):1018-21.

Haruta N, Akamatsu Y, Tsutsui Y, Kurokawa Y, Murayama Y, Arcangioli B, Iwasaki H.
Fission yeast Swi5 protein, a novel DNA recombination mediator.
DNA Repair (Amst). 2008 Jan 1;7(1):1-9.

Akamatsu Y, Tsutsui Y, Morishita T, Siddique MS, Kurokawa Y, Ikeguchi M, Yamao F, Arcangioli B, Iwasaki H.
Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.
EMBO J. 2007 Mar 7;26(5):1352-62.

Haruta N, Kurokawa Y, Murayama Y, Akamatsu Y, Unzai S, Tsutsui Y, Iwasaki H.
The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.
Nat Struct Mol Biol. 2006 Sep;13(9):823-30.


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  氏名:村山 泰斗 (2008.3.博士号取得@横浜市立大学)
  論文タイトル:Analysis of Schizosaccharomyces pombe recombinase Rhp51 in formation of Holliday junctions
  発表論文:
 

Murayama Y, Tsutsui Y, Iwasaki H.
The fission yeast meiosis-specific Dmc1 recombinase mediates formation and branch migration of Holliday junctions by preferentially promoting strand exchange in a direction opposite to that of Rad51.
Genes Dev. 2011 Mar 1;25(5):516-27.

Kuwabara N, Hashimoto H, Yamada N, Unzai S, Ikeguchi M, Sato M, Murayama Y, Iwasaki H, Shimizu T.
Expression, purification and crystallization of Swi5 and the Swi5-Sfr1 complex from fission yeast.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt 9):1124-6. Epub 2010 Aug 31.

Kurokawa Y, Murayama Y, Haruta-Takahashi N, Urabe I, Iwasaki H.
Reconstitution of DNA Strand Exchange Mediated by Rhp51 Recombinase and Two Mediators.
PLoS Biol. 2008 Apr 15;6(4):e88

Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K, Iwasaki H.
Molecular characterization of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double strand break repair in association with the Mre11-Rad50-Nbs1 complex.
Mol Cell Biol. 2008 Jun;28(11):3639-51. Epub 2008 Mar 31.

Murayama Y, Kurokawa Y, Mayanagi K, Iwasaki H.
Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases.
Nature. 2008 Feb 21;451(7181):1018-21.

Haruta N, Akamatsu Y, Tsutsui Y, Kurokawa Y, Murayama Y, Arcangioli B, Iwasaki H.
Fission yeast Swi5 protein, a novel DNA recombination mediator.
DNA Repair (Amst). 2008 Jan 1;7(1):1-9.

Haruta N, Kurokawa Y, Murayama Y, Akamatsu Y, Unzai S, Tsutsui Y, Iwasaki H.
The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.
Nat Struct Mol Biol. 2006 Sep;13(9):823-30.


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  氏名:森 沙織 (2007.8.博士号取得@横浜市立大学)
  論文タイトル:減数分裂特異的な転写による複製開始点の抑制
  発表論文:
 

Mori S, Shirahige K.
Perturbation of the activity of replication origin by meiosis-specific transcription.
J Biol Chem. 2007 Feb 16;282(7):4447-52.

Petronczki M, Matos J, Mori S, Gregan J, Bogdanova A, Schwickart M, Mechtler K, Shirahige K, Zachariae W, Nasmyth K.
Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1.
Cell. 2006 Sep 22;126(6):1049-64.

Riedel CG, Katis VL, Katou Y, Mori S, Itoh T, Helmhart W, Galova M, Petronczki M, Gregan J, Cetin B, Mudrak I, Ogris E, Mechtler K, Pelletier L, Buchholz F, Shirahige K, Nasmyth K.
Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.
Nature. 2006 May 4;441(7089):53-61.

Katis VL, Matos J, Mori S, Shirahige K, Zachariae W, Nasmyth K.
Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis.
Curr Biol. 2004 Dec 29;14(24):2183-96.

Lengronne A, Katou Y, Mori S, Yokobayashi S, Kelly GP, Itoh T, Watanabe Y, Shirahige K, Uhlmann F.
Cohesin relocation from sites of chromosomal loading to places of convergent transcription.
Nature. 2004 Jul 29;430(6999):573-8.


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  氏名:赤松 由布子 (2004.3.博士号取得@大阪大学)
  論文タイトル:分裂酵母における新規組換え修復経路の研究
  発表論文:
 

Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K, Iwasaki H.
Molecular characterization of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double strand break repair in association with the Mre11-Rad50-Nbs1 complex.
Mol Cell Biol. 2008 Jun;28(11):3639-51. Epub 2008 Mar 31.

Akamatsu Y, Tsutsui Y, Morishita T, Siddique MS, Kurokawa Y, Ikeguchi M, Yamao F, Arcangioli B, Iwasaki H.
Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.
EMBO J. 2007 Mar 7;26(5):1352-62.

Haruta N, Kurokawa Y, Murayama Y, Akamatsu Y, Unzai S, Tsutsui Y, Iwasaki H.
The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.
Nat Struct Mol Biol. 2006 Sep;13(9):823-30.

Kato A, Akamatsu Y, Sakuraba Y, Inoue H.
The Neurospora crassa mus-19 gene is identical to the qde-3 gene, which encodes a RecQ homologue and is involved in recombination repair and postreplication repair.
Curr Genet. 2004 Feb;45(1):37-44.

Akamatsu Y, Dziadkowiec D, Ikeguchi M, Shinagawa H, Iwasaki H.
Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast.
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15770-5.

Ueno M, Nakazaki T, Akamatsu Y, Watanabe K, Tomita K, Lindsay HD, Shinagawa H, Iwasaki H.
Molecular characterization of the Schizosaccharomyces pombe nbs1+ gene involved in DNA repair and telomere maintenance.
Mol Cell Biol. 2003 Sep;23(18):6553-63.

Tomita K, Matsuura A, Caspari T, Carr AM, Akamatsu Y, Iwasaki H, Mizuno K, Ohta K, Uritani M, Ushimaru T, Yoshinaga K, Ueno M.
Competition between the Rad50 complex and the Ku heterodimer reveals a role for Exo1 in processing double-strand breaks but not telomeres.
Mol Cell Biol. 2003 Aug;23(15):5186-97.


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  氏名:加藤 由起 (2004.3.博士号取得@横浜市立大学)
  論文タイトル:S期チェックポイントタンパク質Tof1とMrc1は安定な複製停止複合体を形成する
  発表論文:
  Katou Y, Kanoh Y, Bando M, Noguchi H, Tanaka H, Ashikari T, Sugimoto K,
Shirahige K.
S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex.
Nature. 2003 Aug 28;424(6952):1078-83.

Marin A, Gallardo M, Kato Y, Shirahige K, Gutierrez G, Ohta K, Aguilera A.
Relationship between G+C content, ORF-length and mRNA concentration in Saccharomyces cerevisiae.
Yeast. 2003 Jun;20(8):703-11.


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  氏名:高畑 信也 (2004.3.博士号取得@横浜市立大学)
  論文タイトル:TFIID複合体内においてTBPのDNA結合活性を自律的に制御するTAF1 N末端領域(TAND)の機能解析
  発表論文:
 

Mal TK, Takahata S, Ki S, Zheng L, Kokubo T, Ikura M.
Functional Silencing of TATA-binding Protein (TBP) by a Covalent Linkage of the N-terminal Domain of TBP-associated Factor 1.
J Biol Chem. 2007 Jul 27;282(30):22228-38.

Takahata S, Kasahara K, Kawaichi M, Kokubo T.
Autonomous function of the amino-terminal inhibitory domain of TAF1 in transcriptional regulation.
Mol Cell Biol. 2004 Apr;24(8):3089-99.

Takahata S, Ryu H, Ohtsuki K, Kasahara K, Kawaichi M, Kokubo T.
Identification of a novel TATA element-binding protein binding region at the N terminus of the Saccharomyces cerevisiae TAF1 protein.
J Biol Chem. 2003 Nov 14;278(46):45888-902


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  氏名:小林 晶子 (2002.3.博士号取得@奈良先端科学技術大学院大学)
  論文タイトル:TAND欠失型taf145遺伝子と合成致死性を示すNSL遺伝子群の単離と解析
  発表論文:
 

Kobayashi A, Miyake T, Kawaichi M, Kokubo T.
Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP).
Nucleic Acids Res. 2003 Feb 15;31(4):1261-74.

Kobayashi A, Akasaka K, Kawaichi M, Kokubo T.
Functional interaction between TATA and upstream CACGTG elements regulates the temporally specific expression of Otx mRNAs during early embryogenesis of the sea urchin, Hemicentrotus pulcherrimus.
Nucleic Acids Res. 2002 Jul 15;30(14):3034-44.

Kobayashi A, Miyake T, Ohyama Y, Kawaichi M, Kokubo T.
Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae.
J Biol Chem. 2001 Jan 5;276(1):395-405.


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  氏名:月橋 美博 (2001.3.博士号取得@奈良先端科学技術大学院大学)
  論文タイトル:温度感受性を示す変異型TAF145遺伝子の作製とその性質
  発表論文:
 

Tsukihashi Y, Kawaichi M, Kokubo T.
Requirement for yeast TAF145 function in transcriptional activation of the RPS5 promoter that depends on both core promoter structure and upstream activating sequences.
J Biol Chem. 2001 Jul 13;276(28):25715-26.

Tsukihashi Y, Miyake T, Kawaichi M, Kokubo T.
Impaired core promoter recognition caused by novel yeast TAF145 mutations can be restored by creating a canonical TATA element within the promoter region of the TUB2 gene.
Mol Cell Biol. 2000 Apr;20(7):2385-99.

Kotani T, Miyake T, Tsukihashi Y, Hinnebusch AG, Nakatani Y, Kawaichi M, Kokubo T.
Identification of highly conserved amino-terminal segments of dTAFII230 and yTAFII145 that are functionally interchangeable for inhibiting TBP-DNA interactions in vitro and in promoting yeast cell growth in vivo.
J Biol Chem. 1998 Nov 27;273(48):32254-64.


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  氏名:小谷 公泰 (2001.3.博士号取得@奈良先端科学技術大学院大学)
  論文タイトル:TFIIDサブユニット中に存在するTBP機能阻害活性に関する研究
  発表論文:
 

Kotani T, Banno K, Ikura M, Hinnebusch AG, Nakatani Y, Kawaichi M, Kokubo T.
A role of transcriptional activators as antirepressors for the autoinhibitory activity of TATA box binding of transcription factor IID.
Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7178-83.

Kotani T, Miyake T, Tsukihashi Y, Hinnebusch AG, Nakatani Y, Kawaichi M, Kokubo T.
Identification of highly conserved amino-terminal segments of dTAFII230 and yTAFII145 that are functionally interchangeable for inhibiting TBP-DNA interactions in vitro and in promoting yeast cell growth in vivo.
J Biol Chem. 1998 Nov 27;273(48):32254-64.

Kotani T, Zhang X, Schiltz RL, Ogryzko VV, Howard T, Swanson MJ, Vassilev A, Zhang H, Yamauchi J, Howard BH, Qin J, Nakatani Y.
TBP-associated factors in the PCAF histone acetylase complex.
Cold Spring Harb Symp Quant Biol. 1998;63:493-9.

Vassilev A, Yamauchi J, Kotani T, Prives C, Avantaggiati ML, Qin J, Nakatani Y.
The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily.
Mol Cell. 1998 Dec;2(6):869-75.

Ogryzko VV, Kotani T, Zhang X, Schiltz RL, Howard T, Yang XJ, Howard BH, Qin J, Nakatani Y.
Histone-like TAFs within the PCAF histone acetylase complex.
Cell. 1998 Jul 10;94(1):35-44.


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