|Molecular and Cellular Biology Laboratory (Kokubo & Takai Group)
Graduated from the University of Tokyo with a Ph.D.degree (1990).
Postdoctoral fellow at National Institutes of Health, USA (1990-1995).
Associate professor at Nara Institute of Science and Technology
Professor at Yokohama City University (2001-).
Functional analysis of TFIID subunits (TAFs)
The regulatory mechanisms that govern gene expression are of fundamental
importance for understanding the normal processes of various biological
phenomena, including development, differentiation and morphogenesis as
well as for deciphering the molecular basis of various genetic
disorders. The general transcription factor TFIID, which is comprised
of TBP and a series of TAFs, plays a central role in the assembly of the
preinitiation complex as well as in the response to transcriptional
activators. We are currently focusing on the characterization of TAF
function, which is essential for these two molecular events, using
budding yeast as our model organism.
3. Recent publications
Watanabe K, Kokubo T
SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae.
PLoS One. 2017 Nov 27;12(11):e0188435.
Watanabe K, Yabe M, Kasahara K, Kokubo T
A random screen using a novel reporter assay system reveals a set of sequences that are preferred as the TATA or TATA-like elements in the CYC1 promoter of Saccharomyces cerevisiae.
PLoS One. 2015 Jun 5;10(6):e0129357.
Kasahara K, Ohyama Y, Kokubo T
Hmo1 directs pre-initiation complex assembly to an appropriate site on its target
gene promoters by masking a nucleosome-free region.
Nucleic Acids Res. 2011 May;39(10):4136-50.
Sugihara F, Kasahara K, Kokubo T
Highly redundant function of multiple AT-rich sequences as core promoter elements
in the TATA-less RPS5 promoter of Saccharomyces cerevisiae.
Nucleic Acids Res. 2011 Jan 1;39(1):59-75..
Ohyama Y, Kasahara K, Kokubo T
Saccharomyces cerevisiae Ssd1p promotes CLN2 expression by binding to the
5'-untranslated region of CLN2 mRNA.
Genes Cells. 2010 Dec;15(12):1169-88.
Ohtsuki K, Kasahara K, Shirahige K, Kokubo T
Genome-wide localization analysis of a complete set of Tafs reveals a specific effect
of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different
TFIID conformations at different promoters.
Nucleic Acids Res. 2010 Apr 1;38(6):1805-20.
Takahashi H, Kasahara K, Kokubo T
Saccharomyces cerevisiae Med9 comprises two functionally distinct domains that play
different roles in transcriptional regulation.
Genes Cells. 2009 Jan;14(1):53-67.
Kasahara K, Ki S, Aoyama K, Takahashi H, Kokubo T.
Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in
start site selection by RNA polymerase II.
Nucleic Acids Res. 2008 Mar;36(4):1343-57.