生殖再生医学・in vitro精子形成・小川毅彦研究室

発表論文

2019年

Komeya M, Yamanaka H., Sanjo H, Yao M, Nakamura H, Kimura H, Fujii T, Sato T, Ogawa T. In vitro spermatogenesis in two-dimensionally-spread mouse testis tissues. Reprod Med Biol. 2019 Oct; 18(4): 362-369.

Ogawa T. Live Offspring after Testis Tissue Transplantation. N Engl J Med. 2019 Oct 10;381(15):1477-1479.

Fukunaga H, Kaminaga K, Sato T, Butterworth KT, Watanabe R, Usami N, Ogawa T, Yokoya A, Prise KM. High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation. Sci Rep. Oct 1;9(1):12618. (2019)

Sato T, Ogawa T. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method. Methods Mol Biol. 1874:87-98 (2019)

2018年

Komeya M, Sato T, Ogawa T. In vitro spermatogenesis: A century-long research journey, still half way around. Reprod Med Biol. 2018 Aug 12;17(4):407-420.

Kojima K, Nakamura H., Komeya M., Yamanaka H., Makino Y., Okada Y. Akiyama, H., Torikai N., Sato T., Fujii T., Kimura H. and Ogawa T. Neonatal testis growth recreated in vitro by two-dimensional organ-spreading. Biotechnol Bioeng. doi: 10.1002/bit.26822. (2018).

Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T. A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility. Biochem Biophys Res Commun. 500: 885-891 (2018).

Fukunaga, H., Kaminaga, K., Sato, T., Usami, N., Watanabe, R., Butterworth, K., Ogawa, T., Yokoya, A. and Prise, K. Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis. RADIATION RESEARCH 189, 661–667 (2018)

Sanjo H, Komeya M, Sato T, Abe T, Katagiri K, Yamanaka H, Ino Y, Arakawa N, Hirano H, Yao T, Asayama Y, Matsuhisa A, Yao M, , Ogawa T. In vitro mouse spermatogenesis with an organ culture method in chemically defined medium. PLos One. Feb 12;13(2):e0192884 (2018)

2017年

Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T. Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro. Sci Rep. Nov 13;7(1):15459 (2017)

Nakajima R, Sato T, Ogawa T, Okano H, Noce T. A noncoding RNA containing a SINE-B1 motif associates with meiotic metaphase chromatin and has an indispensable function during spermatogenesis. PLoS One Jun 28;12(6):e0179585. (2017)

Nakamura N, Merry GE, Inselman AL, Sloper DT, Del Valle PL, Sato T, Ogawa T, Hansen DK. Evaluation of Culture Time and Media in an In Vitro Testis Organ Culture System. Birth defects research. doi:10.1002/bdr2.1002 (2017)

Tokue M, Ikami K, Mizuno S, Takagi C, Miyagi A, Takada R, Noda C, Kitadate Y, Hara K, Mizuguchi H, Sato T, Taketo MM, Sugiyama F, Ogawa T, Kobayashi S, Ueno N, Takahashi S, Takada S, Yoshida S. SHISA6 Confers Resistance to Differentiation-Promoting Wnt/beta-Catenin Signaling in Mouse Spermatogenic Stem Cells. Stem Cell Reports 8 (3):561-575. doi:10.1016/j.stemcr.2017.01.006 (2017)

2016年

Kojima K, Sato T, Naruse Y, Ogawa T. Spermatogenesis in Explanted Fetal Mouse Testis Tissues. Biol Reprod. 95(3); 63 (2016).

Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T. Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device. Sci Rep. Feb 19; 6: 21472. (2016)

2015年

Sato T, Sakuma T, Yokonishi T, Katagiri K, Kamimura S, Ogonuki N, Ogura A, Yamamoto T, Ogawa T. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9. Stem Cell Reports Jul 14; 5(1): 75-82. (2015)

Sato T,Katagiri K, Kojima K, Komeya M, Yao M, Ogawa T. In Vitro Spermatogenesis in Explanted Adult Mouse Testis Tissues. PlosONE. Jun 12; 10(6): e0130171. (2015)

2014年
Yokonishi T, Sato T, Komeya M, Katagiri K, Kubota Y, Nakabayashi K, Hata K, Inoue K, Ogonuki N, Ogura A, Ogawa T. Offspring production with sperm grown in vito from cryopreserved testis tissues Nature Communications. 5, 4320. (2014)

2013年
Sato T, Katagiri K, Kubota Y, Ogawa T. In vitro sperm production from mouse spermatogonial stem cell lines using an organ culture method. Nature protoc. 8, 2098-2104. (2013)

Yokonishi T, Sato T, Katagiri K, Komeya M, Kubota Y, Ogawa T. In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation. Biol Reprod. 89(1):15, 1?6 (2013)

Shiura H, Ikeda R, Lee J, Sato T, Ogonuki N, Hirose M, Ogura A, Ogawa T, Abe K. Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse. Genesis 26, 1-8. (2013)

Yokonishi T, Sato T, Katagiri K, Ogawa T. In vitro spermatogenesis using an organ culture technique. Methods Mol Biol. 927, 479-488 (2013).

2012年
Sato T, Yokonishi T, Komeya M, Katagiri K, Kubota Y, Matoba S, Ogonuki N, Ogura A, Yoshida S, Ogawa T. Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice. Proc Natl Acad Sci U S A. 109, 16934-16938 (2012).
プレスリリース


2011年
Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T. In vitro production of fertile sperm from murine spermatogonial stem cell lines. Nature Communications 2, 472 (2011).
プレスリリース

Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T: In vitro production of functional sperm in cultured neonatal mouse testes. Nature 471, 504-507 (2011).
プレスリリース

2010年
Araki Y, Sato T, Katagiri K, Kubota Y, Araki Y, Ogawa T. Proliferation of Mouse Spermatogonial Stem Cells in Microdrop Culture. Biology of Reproduction83: 951-957 (2010).

Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T. In Vitro Murine Spermatogenesis in an Organ Culture System. Biology of Reproduction 83: 261-267 (2010).

2009年以前

著作・総説・解説文

Komeya M, Sato T. Ogawa T. In vitro spermatogenesis: A century‐long research journey, still half way around Reprod Med Biol. 2018;17:407– 420

Ogawa T. (2017) In Vitro Differentiation of Spermatogonia. In: Oatley J., Griswold M. (eds) The Biology of Mammalian Spermatogonia. Springer, New York, NY

佐藤卓也、小川毅彦:佐藤卓也、小川毅彦: 「精子幹細胞研究の展望」臨床婦人科産科 Vol. 69, No.8, 786-790 (2015)

古目谷 暢、小川毅彦:「酸化ストレスと精巣」 腎臓内科・泌尿器科 Vol. 2, 27-33 (2015)

Komeya M, Ogawa T. Spermatogonial stem cells: Progress and prospects. Asian J Androl. (2015)

古目谷 暢、小川毅彦:「精子幹細胞」 Hormone Frontier in Gynecology Vol. 21, 131-136 (2014)

佐藤卓也、横西哲広、小川毅彦:「精子幹細胞と精子形成:ex vivo cultureの可能性」  実験医学 Vol. 32, 859-864 (2014)

横西哲弘、小川毅彦:「精巣組織の凍結保存」 臨床婦人科産科、医学書院 Vol. 68, No. 1, 54-63 (2014)

小川毅彦:「in vitro精子形成法と卵細胞質内精子注入(ICSI)」 J. Mamm.Ova Res. Vol.30 (4), 155-158 (2013)

古目谷 暢、小川毅彦:「精子形成研究の・ォ来」 新版今日の不妊診療 ・髢リ秋悦編集、医歯薬出版株式会社 P303-306 (2013)

佐藤卓也・小川毅彦 「グラビア・目で見る生殖幹細胞」 HORMONE FRONTIER IN GYNECOLOGY vol. 20 188-191 (2013)

Takehiko Ogawa: In vitro production of functional sperm from neonatal mouse testes, Chapter 4 in Stem Cells in Reproductive Medicine, 3rd edition edited by Carlos Simon, Antonio Pellicer, & Renee Reijo Pera. CAMBRIDGE UNIVERSITY PRESS 2013.

佐藤卓也・小川毅彦 「新生仔マウス精巣からの試験管内精子産生」 BIO Clinica vol. 28 80-84 (2013)

横西哲広・古目谷暢・佐藤卓也・小川毅彦 「精巣組織の凍結」 HORMONE FRONTIER IN GYNECOLOGY Vol.20 169-175 (2013)

佐藤卓也・小川毅彦 精子形成不全マウスの精巣組織から培養下での精子産生 細胞工学 Vol.32 No.2 212-213 (2013)

小川毅彦:「精子幹細胞から精子へのin vitro分化誘導」 特集:生殖医療の今日的革新 産婦人科の実際 60, 759-765 (2011)

佐藤卓也、小川毅彦: 「in vitroでマウスの精子幹細胞から妊孕能のある精子を産生することに成功」クローズアップTOPICS 細胞工・w Vol.30 No.9, 980-983, (2011)

小川毅彦;「精子幹細胞のマニピュレーション・テクノロジー 【特集:ニューテクノロジ ―明日の生殖医学研究に向けて】」HORMONE FRONTIER IN GYNECOLOGY 18巻3号 79-84 (2011)

小川毅彦:「哺乳類の精子幹細胞のマニピュレーション:医学的な展望や課題を含めて」 細胞工学 Vol.29, No.7, 687-692(2010)(2010年7月号 特集:配偶子幹細胞) 

2009年以前

学会発表

2010年

                      Cold Spring Harbor symposium 

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横浜市立大学

新学術領域


配偶子産生制御(平成30~令和4年)


配偶子産生制御(平成25~29年)


配偶子幹細胞制御(平成20~24年)